When Miles Morales is unexpectedly approached by his best friend and love interest Gwen Stacy to complete a mission to save every universe of Spider-People from a mysterious new villain who could cause a catastrophic disaster, the Spot, Miles is up for the challenge. He and Gwen journey through the Multiverse together and meet its protectors, a group of Spider-People known as the Spider-Society, led by Miguel O'Hara. However, Miles finds himself at odds with Miguel and the Spider-Society on how to handle the threat.